J Cosmet Med 2020; 4(2): 101-107  https://doi.org/10.25056/JCM.2020.4.2.101
Utilization of lyophilized platelet-rich plasma in esthetic facial treatment
Teh-Yang Cheng, MD
Department of Dermatology, National Taiwan University Hospital, Taipei City, Taiwan
Teh-Yang Cheng
E-mail: jerome.cheng@gmail.com
Teh-Yang Cheng’s current affiliation: Infinite Delight Aesthetic Clinic, Taipei City, Taiwan
Received: August 19, 2020; Revised: September 21, 2020; Accepted: September 21, 2020; Published online: December 31, 2020.
© Korean Society of Korean Cosmetic Surgery. All rights reserved.

cc This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Platelet-rich plasma (PRP) has a variety of functions. Activated platelets release various growth factors and are involved in the tissue repair process. Growth factors from platelets induce fibroblast activation, which produces a new extracellular matrix and collagen. The PRP treatment has been widely used in many surgical and medical fields. However, uncertainties in the quality of the PRP preparation may cause unpredictability in the treatment results. The standardized separation method with quantization of lyophilized platelet-rich plasma (L-PRP) might enhance treatment accuracy. After processing in central laboratory, L-PRP is packed in vials in powder form and readily used immediately after reconstitution. We utilized clinical photography, Visia, DermaLab USB, and histology examination to compare the results before and after treatments. Besides improvements in clinical photos and Visia scores, ultrasound images showed obvious increasing in collagen densities in dermal and subcutaneous layers. The histology study suggested increased collagen formation but without signs of fibrotic change or foreign body reaction. The elastin stain suggested an increased elastic fiber density. The improvement noted by the instruments and histology findings correlated with the improvement of skin texture, decreased wrinkles, and positive clinical outcomes. These results highlight L-PRP’s potential as an efficient and convenient process compared to the original PRP in skin rejuvenation treatment.
Keywords: lyophilized platelet-rich plasma; neo-collagenesis; platelet-rich plasma; skin rejuvenation
  1. Tsay RC, Vo J, Burke A, Eisig SB, Lu HH, Landesberg R. Differential growth factor retention by platelet-rich plasma composites. J Oral Maxillofac Surg 2005;63:521-8.
    Pubmed CrossRef
  2. Dhurat R, Sukesh M. Principles and methods of preparation of platelet-rich plasma: a review and author’s perspective. J Cutan Aesthet Surg 2014;7:189-97.
    Pubmed KoreaMed CrossRef
  3. Peng GL. Platelet-rich plasma for skin rejuvenation: facts, fiction, and pearls for practice. Facial Plast Surg Clin North Am 2019;27:405-11.
    Pubmed CrossRef
  4. Frautschi RS, Hashem AM, Halasa B, Cakmakoglu C, Zins JE. Current evidence for clinical efficacy of platelet rich plasma in aesthetic surgery: a systematic review. Aesthet Surg J 2017;37:353-62.
    Pubmed CrossRef
  5. Mariani E, Pulsatelli L. Platelet concentrates in musculoskeletal medicine. Int J Mol Sci 2020;21:1328.
    Pubmed KoreaMed CrossRef
  6. Shirata T, Kato Y. Can intra-articular injection of freeze-dried platelet-derived factor concentrate regenerate articular cartilage in the knee joint? Regen Ther 2019;11:5-7.
    Pubmed KoreaMed CrossRef
  7. Pierce GF, Mustoe TA, Altrock BW, Deuel TF, Thomason A. Role of platelet-derived growth factor in wound healing. J Cell Biochem 1991;45:319-26.
  8. Verma R, Negi G, Kandwal A, Chandra H, Gaur DS, Harsh M. Effect of autologous PRP on wound healing in dental regenerative surgeries and its correlation with PDGF levels. Asian J Transfus Sci 2019;13:47-53.
    Pubmed KoreaMed CrossRef
  9. da Silva LQ, Montalvão SAL, Justo-Junior ADS, Cunha Júnior JLR, Huber SC, Oliveira CC, et al. Platelet-rich plasma lyophilization enables growth factor preservation and functionality when compared with fresh platelet-rich plasma. Regen Med 2018;13:775-84.
    Pubmed CrossRef
  10. Yeung CY, Hsieh PS, Wei LG, Hsia LC, Dai LG, Fu KY, et al. Efficacy of lyophilised platelet-rich plasma powder on healing rate in patients with deep second degree burn injury: a prospective double-blind randomized clinical trial. Ann Plast Surg 2018;80(2S Suppl 1):S66-9.
    Pubmed CrossRef

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